Haploid Cultures
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چکیده
منابع مشابه
Haploid cells in rat kangaroo corneal endothelium cultures and x-ray-induced chromosome aberrations.
N the reproduction of mammals? germ cells undergo two maturation divisions I to yield haploid gametes which, through union with gametes from the opposite sex, give rise to the next generation. Based on the existence of haploid status in gametes, investigators in the 1940’s and 50’s experimentally induced haploid cells in parthenogenetically developed blastocysts or in very early embryos of rabb...
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Hemizygous mutations introduced in haploid genomes can directly expose a phenotype, thus facilitating gene function analysis and forward genetic screening. Recently, mammalian haploid cells could be derived from mouse, rat, monkey, and human embryos and have been applied to screens of cellular mechanisms including cell signaling, pathogen host factors, and developmental pathways. Notably, haplo...
متن کاملRare-Cell Fusion Events between Diploid and Haploid Strains of the Sexually Agglutinative Yeast HANSENULA WINGEI.
Diploids of the yeast Hansenula wingei are nonagglutinative and do not form zygotes in mixed cultures with either sexually agglutinative haploid mating type. However, a low frequency of diploid x haploid cell fusions (about 10(-3)) is detectable by prototrophic selection. This frequency of rare diploid x haploid matings is not increased after the diploid culture is induced for sexual agglutinat...
متن کاملInvestigations on ploidy levels of haploid and diploid callus and cell suspension cultures of Datura innoxia Mill.
Using haploid and diploid Datura innoxia Mill, callus cultures and cell suspensions it was shown that meristematic material usually attains the lowest possible C-value of a given ploidy level. Parenchyma material of callus cultures, however, indicates a broad scattering of C-values up to 16 C, which is paralleled by C-value distribution of the free cell fraction in actively dividing cell suspen...
متن کاملBiochemical properties of haploid and diploid strains of Penicillium chrysogenum.
An intensive parasexual genetics program in which industrial strains of Penicillium chrysogenum were used culminated in the isolation of a number of heterozygous diploid strains. The diploid clones were selected from heterokaryons formed from matings between mutant strains having complementary biochemical and conidial color markers. Several diploid cultures were compared with their haploid wild...
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